MB 5157 PRACT X-RAY CRYSTALLOGRAPHY
Practical X-ray Crystallography will mix lectures and hands-on tutorials, with the goal of providing beginners in the discipline the tools to move forward confidently on crystallographic projects of their own. In the tutorial section, students will grow protein crystals, collect and process X-ray diffraction data, solve the phase problem using both molecular replacement and anomalous diffraction, build protein models, refine the model, analyze the model, and learn effective model presentation. Students will be tutored in the use of state-of-the-art crystallographic software. In the lectures, the principles behind the methods will be discussed. Prerequisite for this course: Modern methods in structural biology. Recommended prerequisite: Protein Structure and Folding.