MB 5102 MOD METH OF STRUCT BIO
Much of modern structural biology is based on results obtained with two high-resolution methods (X-ray crystallography, NMR spectroscopy), often complemented by several lower-resolution approaches (EM, scattering, FRET, among others). We assert that the successful union of these general approaches is absolutely critical in modern structural biology, particularly as biophysical methods are applied to larger, multicomponent systems that are often dynamic in their composition. This course provides the foundation for students to understand these techniques, extending the introduction provided in the first year Core Course. A central focus for the course will be discussions of both the theory and application of X-ray crystallography and NMR spectroscopy, with the aim to establish the physical bases of both methods using instruction that covers theory and application. Combined with introductions into the lower-resolution methods, this course will provide students with the ability to critically evaluate the relative strengths and weaknesses of each technique and how they can be combined to provide insight into biological systems.