CMB 5103 LIGHT AND ELECTRON MICROSCOPY: FROM FUNDAMENTALS TO CUTTING-EDGE APPLICATIONS

During the first part of these 90-min lectures, the course addresses the principles and applications of modern optical microscopy, including among others: properties of light, anatomy of light microscopes, digital imaging, quantitative fluorescence microscopy, image analysis/quantification and cutting-edge technologies. The second part revolves around the fundamentals and applications of electron microscopy in biological research, including, conventional EM techniques, high-pressure freezing/freeze substitution, cryo-electron microscopy and tomography, and correlative techniques.

Credits

1.5