MB 5106 USING LIGHT IN BIOLOGY
Overview of optical spectroscopic approaches to biological systems, both in vitro and via light microscopy of cells. Begins with discussion of the interaction of light with matter, and extension to absorption spectroscopy, and UV, both visible and IR. Circular dichroism of proteins and other chromophores. Fluorescence and fluorescence-based techniques. Static and dynamic light scattering. The course intends to develop physical principles to support rigorous biophysical applications and experimental design.